Partial Purification and Characterization of β-D-Galactosidase from Kluyveromyces marxianus
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چکیده
منابع مشابه
Electroporation of Kluyveromyces marxianus and -D-galactosidase Extraction
Nowadays electroporation is well established as a method for increasing the permeability of biological membranes aiming to include some kind of molecule inside cells (anticancer drugs, for example) or to extract molecules from cells (enzymes, DNA, etc.). The permeabilized cells show a distribution of hydrophilic pores with diameters of several nanometers in the regions where the induced transme...
متن کاملPARTIAL PURIFICATION AND CHARACTERIZATION OF B-GALACTOSIDASE FROM ASPERGILLUS NIGER UV-5
The enzyme pgalactosidase from a mutant strain of A. niger UV-5 was partially purified using ammonium sulfate and acetone. The saturation range of 60-80% ammonium sulfate was found to yield 60.5% enzyme recovery with 2.4 fold purification. Acetone precipitation at enzyme: acetone ratio of 1 : 1.5 brought about a higher yield i.e. 68% and three-fold purification. The combined procedures of ...
متن کاملKinetics and Regulation Studies of the Production of -Galactosidase from Kluyveromyces marxianus Grown on Different Substrates
Lactose-intolerance is manifested in 50 % of the world’s population. This can be remediated by removing lactose from the diet or converting it into glucose and galactose with -galactosidase (EC 3.2.1.23). In this work, batch production of this enzyme in the presence of lactose, galactose, cellobiose, xylose, arabinose, sucrose and glucose was investigated using Kluyveromyces marxianus in shake ...
متن کاملpartial purification and characterization of b-galactosidase from aspergillus niger uv-5
the enzyme pgalactosidase from a mutant strain of a. niger uv-5 was partially purified using ammonium sulfate and acetone. the saturation range of 60-80% ammonium sulfate was found to yield 60.5% enzyme recovery with 2.4 fold purification. acetone precipitation at enzyme: acetone ratio of 1 : 1.5 brought about a higher yield i.e. 68% and three-fold purification. the combined procedures of 1.5 v...
متن کاملCharacterization of Yeast Protein Enzymatic Hydrolysis and Autolysis in Saccharomyces cerevisiae and Kluyveromyces marxianus
Protein recovery under sonication treatment and autolysis, also protein hydrolysis progress during enzymatic hydrolysis (using trypsin and chymotrypsin) and autolysis (using endogenous enzymes) were investigated in Saccharomyces cerevisiae and Kluyveromyces marxianus. Crude protein content of dried yeast cells were 53.22% and 45.6% for S.cerevisiae and K.marxianus, respectively. After 96 hrs of...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1982
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(82)82466-1